Jamestown Canyon Virus: Reporting and Surveillance

Jamestown Canyon virus is a category II reportable condition in Wisconsin. Health care providers and laboratories should report to the patient's local public health department:
  • Electronically through the Wisconsin Electronic Disease Surveillance System (WEDSS).
  • By calling the Bureau of Communicable Diseases at 608-267-9003.

Reporting should be completed within 72 hours upon recognition of a case. For more information, please visit the DHS Disease Reporting page.

Watch our Arboviral Disease Surveillance Update Webinar to learn about arboviral diseases in Wisconsin, prevention and control, and surveillance and reporting information and updates. Recorded on July 14, 2017.

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 Guidance Resources

DHS Surveillance Resources


Laboratory diagnosis of Jamestown Canyon virus most often involves the detection of antibodies against Jamestown Canyon virus in serum or cerebrospinal fluid (CSF). Diagnostic testing for Jamestown Canyon virus is not widely available at commercial laboratories. An arbovirus IgM antibody panel can be requested through the Wisconsin State Laboratory of Hygiene (WSLH), which includes testing for West Nile, St. Louis encephalitis, Jamestown Canyon, La Crosse encephalitis, Eastern equine encephalitis, and Powassan viruses. If Jamestown Canyon virus infection is suspected, concurrent testing for other domestic arboviruses should be considered, since clinical features of these viruses overlap.

The WSLH arbovirus IgM antibody panel screens for evidence of infection through IgM antibody capture enzyme-linked immunosorbent assay or microsphere immunoassay performed on serum or CSF. The WSLH will forward specimens with positive IgM results to CDC for confirmatory testing by plaque reduction neutralization test (PRNT).

The arbovirus IgM antibody panel is available at the WSLH fee-for-service. It is not required that patients’ symptoms meet the Division of Public Health (DPH) clinical criteria to submit serum or CSF specimens to the WSLH for fee-for-service arboviral screening.

DPH monitors for Jamestown Canyon virus as part of our enhanced arbovirus surveillance. Testing for Jamestown Canyon virus as part of a DPH investigation may be fee-exempt. Please contact a vectorborne disease epidemiologist at 608-267-9003 for fee-exempt approval.

Fee-exempt arboviral antibody testing will be offered to clinicians whose patients meet ONE of the following criteria:

  • Request for confirmatory testing of positive arboviral IgM and IgG commercial test results (performed at laboratories other than the WSLH).
  • Jamestown Canyon virus is the suspected agent causing illness and testing for other arboviruses (West Nile, La Crosse, St. Louis, Powassan, and Eastern equine encephalitis) demonstrated negative IgM antibody results.
  • The patient has signs and symptoms of meningitis (fever, headache, and stiff neck) or encephalitis (fever, headache, and altered mental status ranging from confusion to coma), or acute flaccid paralysis (AFP) with no other laboratory diagnosis.
  • The patient has a diagnosis of Guillain-Barré syndrome and no other laboratory diagnosis.
  • The local health department may request fee-exempt testing be performed if the case-patient lacks insurance coverage or the ability to pay.

Instructions for collection and shipping of clinical specimens to the WSLH:

  • Collect specimens between three days and 12 weeks after symptom onset for reliable antibody detection. Samples collected less than eight days after illness onset may not demonstrate IgM reactivity due to the time required for the development of an antibody response, and a convalescent sample may be indicated.
  • Submit 3-7 mL of serum and/or >1 mL of CSF in sterile screw-capped vials on cold packs using a WSLH kit #22 or equivalent within five days of collection. It is recommended that a paired serum specimen be submitted with a CSF specimen. Specimens should be triple packaged as a Category B Biological Substance (include UN3373 label).
  • Fee-exempt testing submissions must include a WSLH Enhanced Wisconsin Arbovirus Surveillance form provided by a DPH vectorborne disease epidemiologist.
  • Fee-for-service testing submissions must include a WSLH CDD Requisition Form B. Please contact the WSLH Clinical Orders at 800-862-1088 or 608-224-4275 to obtain blank copies of WSLH CDD Requisition Form B and to order specimen shipping kits.
  • It is essential that the lab requisition form be as complete as possible, including patient name, city, date of birth, specimen type, submitting agency, onset date, signs and symptoms, and collection date. Note: Testing for Jamestown Canyon virus may be delayed on specimens missing the above data until information is available.
  • Ship specimens with appropriate completed form to:

Wisconsin State Laboratory of Hygiene
2601 Agriculture Drive,
PO Box 7904
Madison, WI 53718
Customer Service: 800-862-1013


Diagnosis of Jamestown Canyon virus can be challenging since many of the signs and symptoms of Jamestown Canyon virus can be similar to other viral infections. Furthermore, Jamestown Canyon virus IgM antibody cross-reactivity can occur among related viruses, complicating timely diagnosis. A patient's clinical signs and symptoms, travel history in an area with epidemiologic history of Jamestown Canyon virus, and risk activities can help to inform a preliminary diagnosis prior to laboratory diagnosis.


There is currently no treatment or vaccine for Jamestown Canyon virus. Over-the-counter pain relievers may be given to relieve symptoms. In severe cases, patients may need to be hospitalized to receive supportive treatment.

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Phone: 608-267-9003 | Fax: 608-261-4976

Last Revised: January 20, 2021